Description
Overview
The Cyto-Cellect® Human IgG Kappa and Viability Assay Kit is a multiplex fluorescent assay designed for use Cyto-Mine® platforms. It enables high-throughput screening by detecting single-cell antibody secretion while simultaneously assessing cell viability, ensuring the selection of only viable, high-producing cells.
CO-ENCAPSULATION: Individual cells and fluorescent assay probes are encapsulated in picodroplets inside Cyto-Mine® systems.
SIMULTANEOUS DETECTION: The assay quantifies antibody secretion and assesses viability within the same picodroplet, distinguishing between high-producing, viable clones and apoptotic or dead cells.
EFFICIENT SELECTION: Non-viable cells are excluded from the workflow, ensuring that only healthy and productive clones progress to downstream development.
Who is it for?
- Cell Line Development (CLD) Teams – Select high-yielding, stable clones with confidence.
- CDMOs & BioPharma Companies – Accelerate therapeutic development.
- Existing Cyto-Mine® platform users – Expanding capabilities with next-level multiplex assays.
How it works
The fluorescent assay probes co-encapsulate with individual cells into picodroplets within Cyto-Mine® platforms. Inside the picodroplets, these probes detect Human IgG Kappa secretion using Förster resonance energy transfer (FRET), while simultaneously detecting apoptotic markers by Annexin V staining. These fluorescent signals are used to sort and isolate high-producing, viable clones for cell line development workflows.
Features
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Multiplex Detection: Simultaneously screen for antibody secretion and cell viability
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Apoptotic Cell Exclusion: Actively remove dying or non-viable cells during screening and prevent false positives.
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High-Throughput Screening: Process up to 300,000 cells per experiment
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Optimized for Cyto-Mine® Chroma: Seamless integration for automated, single-step workflows
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Simple Assay Setup: Ready-to-use reagents for a straightforward workflow
Benefits
Reduce screening uncertainty by confirming secretion and viability at once. You get higher-quality hits and fewer false positives for follow up.
Improve downstream outcomes by ensuring only healthy, productive cells are selected. Minimize wasted time on non-viable clones and increase confidence in early-stage decisions.
Boost statistical power and rare hit recovery. Screen larger populations to uncover top performers faster, even in low-frequency events.
Unlock full platform performance. Run end-to-end assays without manual steps, freeing up your team for higher-priority tasks.
Spend less time troubleshooting, more time discovering. Validated reagents reduce setup complexity and give consistent results across users and sites.
Discover the
Cyto-Cartridge®
The Cyto-Cartridge® is the core of the Cyto-Mine® platform, providing all the necessary microfluidics for implementing picodroplet workflows.
The disposable cartridge is designed to be easy-to-use, ensuring zero cross-contamination between runs and maintaining the integrity and quality of your cells for each experiment.
Cyto-Cartridges® are manufactured from fully biocompatible materials under our ISO 9001 quality management system, in a clean environment.
