Description
Overview
The Cyto-Cellect® Human IgG Kappa and Viability Assay Kit is a multiplex fluorescent assay designed for use with Cyto-Mine® Chroma. It enables high-throughput screening by detecting single-cell antibody secretion while simultaneously assessing cell viability, ensuring the selection of only viable, high-producing cells.
Who is it for?
The Cyto-Cellect® Human IgG Kappa and Viability Assay Kit is ideal for:
- Cell Line Development (CLD) Teams – Select high-yielding, stable clones with confidence.
- CDMOs & BioPharma Companies – Accelerate therapeutic development.
- Existing Cyto-Mine® Chroma users – Expanding capabilities with next-level multiplex assays.
How it works
The fluorescent assay probes co-encapsulate with individual cells into picodroplets within the Cyto-Mine® Chroma system. Inside the picodroplets, these probes detect Human IgG Kappa secretion using Förster resonance energy transfer (FRET) probes, while simultaneously detecting apoptotic markers by Annexin V staining. These fluorescent signals are used to sort and isolate high-producing, viable clones for cell line development workflows.
Features
Multiplex Detection – Simultaneously screen for antibody secretion and cell viability
Apoptotic Cell Exclusion – Prevent false positives by eliminating non-viable cells
High-Throughput Screening – Process up to 300,000 cells per experiment
Optimized for Cyto-Mine® Chroma – Seamless integration for automated, single-step workflows
Simple Assay Setup – Ready-to-use reagents for a straightforward workflow
Benefits
Recover only high-producing, viable clones – Increase the likelihood of stable, high-yielding clones for cell line development.
Eliminate false positives – Prevent apoptotic cells from skewing results, ensuring a more reliable selection process.
Accelerate workflows & reduce costs – Minimize time spent on non-viable clones, streamlining the transition from screening to scale-up.
Maximize discovery potential – Screen more single cells than competing technologies in a fully automated, hands-free process.
Discover the Cyto-Cartridge®
The Cyto-Cartridge® is the core of the Cyto-Mine® platform, providing all the necessary microfluidics for implementing picodroplet workflows.
The disposable cartridge is designed to be easy-to-use, ensuring zero cross-contamination between runs and maintaining the integrity and quality of your cells for each experiment.
Cyto-Cartridges® are manufactured from fully biocompatible materials under our ISO 9001 quality management system, in a clean environment.
