Description
Overview
Confidently pinpoint your top-producing clones—fast. Cyto-Cellect® Plus is a ready-to-use, FRET-based assay that detects secreted human IgG with kappa (κ) and lambda (λ) light chains at the single-cell level. Designed for seamless integration with Cyto-Mine® technology, the assay co-encapsulates fluorescent probes with cells inside picodroplets. As secretion occurs, the probes bind to the antibody in close proximity, generating a measurable FRET signal. The result? Rapid, high-sensitivity insights into productivity—so you can move forward with confidence.
Who is it for?
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Cell Line Development (CLD) Teams – Select high-yielding, stable clones with confidence.
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CDMOs & BioPharma Companies – Accelerate therapeutic development.
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Existing Cyto-Mine® platform users – Expanding capabilities with next-level multiplex assays.
How it works
Cyto-Cellect® Plus uses a FRET-based detection mechanism to quantify IgG secretion from individual cells. During Cyto-Mine® platform operation, each cell is encapsulated in a picodroplet alongside kappa and lambda-specific fluorescent probes. When a cell secretes IgG, the probes bind in close proximity, forming a ternary complex. This interaction alters the FRET signal, which is detected by the Cyto-Mine® laser system. The signal decreases as antibody concentration increases, enabling precise, high-throughput measurement of cell productivity.
A customized pair of IgG-specific AOF fluorescent probes are trapped within each picodroplet. IgG secreted from the encapsulated cell is recognized by the detection probe pair forming a ternary FRET complex that induces a change in fluorescent signal.
Features
- Dual Light Chain Detection (IgGκ and IgGλ)
Accurately detect and quantify human IgG molecules with κ and λ light chains in a single assay. - FRET-Based Assay for Secreted Antibodies
Enables sensitive, real-time detection of antibody secretion from single cells encapsulated in picodroplets. - Optimized for Cyto-Mine® Platform Workflows
Seamless compatibility with CHO culture media and Cyto-Mine® systems—no assay customization needed. - Animal-Origin-Free (AOF) Reagents
Manufactured without human- or animal-derived materials to meet regulatory and quality expectations. - Workflow-Ready Reagent Kit
Includes 10 pre-mixed vials (5 donor / 5 acceptor) for plug-and-play assay setup
Benefits
- Confident Clone Selection.
Detect only cells secreting complete antibodies—boosting reliability in selecting high-producing clones.
- Accelerated Development Timelines.
Streamline screening and shorten time-to-decision with high-throughput, single-cell resolution data.
- Assay Simplicity = Faster Startup
Skip lengthy optimization—validated probes work out of the box, reducing setup time and variability.
- Improved Compliance Readiness.
AOF formulation helps support traceability and regulatory alignment for GMP and CDMO partners.
- Greater Insight, Less Reagent Waste
Multiplex detection in nanoliter volumes provides more data per cell while minimizing assay costs.
Discover the
Cyto-Cartridge®
The Cyto-Cartridge® is the core of the Cyto-Mine® platform, providing all the necessary microfluidics for implementing picodroplet workflows.
The disposable cartridge is designed to be easy-to-use, ensuring zero cross-contamination between runs and maintaining the integrity and quality of your cells for each experiment.
Cyto-Cartridges® are manufactured from fully biocompatible materials under our ISO 9001 quality management system, in a clean environment.
