Description
Overview
Cyto-Cellect® Fc Fusion provides a fluorescent assay that allows users to directly detect and select the encapsulated cells that produce the highest amounts of Fc fusion proteins during Cyto-Mine® platform experiments. The kit enables the detection of secreted human Fc fusion proteins from one or more cells per picodroplet. This kit is capable of analyzing up to 400,000 single cells per run and up to 40 million cells for pooled cell analysis. productivity, increasing the chances of finding rare, high-quality clones.
The assay probes are manufactured without the use of animal- or human-derived materials. Our detection kit is workflow-ready and compatible with CHO cell culture medium, enabling you to start your experiments with minimal effort.
Who is it for?
- Cell Line Development (CLD) Teams – Select high-yielding, stable clones with confidence.
- CDMOs & BioPharma Companies – Accelerate therapeutic development.
- Existing Cyto-Mine® platform users – Expanding capabilities with next-level multiplex assays.
How it works
This FRET-based competition assay can be used to detect human Fc fusion proteins containing human IgG Fc region. It has been designed to work in conjunction with Cyto-Mine® technology to detect secreted recombinant human Fc fusion proteins from cells encapsulated within picodroplets. This assay kit shows a decreasing FRET signal with increasing concentrations of the target protein.
Schematic diagram of the Cyto-Cellect® Fc Fusion detection kit for Fc fusion protein secreting cells.
Features
- FRET-Based Competition Assay
Detect secreted Fc fusion proteins with high sensitivity using a validated Förster resonance energy transfer (FRET) format. - Single-Cell Resolution
Screen individual cells in picodroplets to directly identify the top Fc fusion producers. - Quantitative Protein Detection
FRET signal intensity correlates to Fc protein concentration, enabling precise productivity comparisons. - Ultra-High-Throughput Capability
Analyze up to 400,000 individual cells or pool up to 40 million in a single experiment. - Plug-and-Play Integration with Cyto-Mine® Technology
Pre-optimized formulation ensures seamless use across all Cyto-Mine® platforms. - Animal-Origin-Free (AOF)
All reagents are manufactured without human- or animal-derived components to support GMP workflows and regulatory compliance.
Benefits
Confidently Select High-Producers
Eliminate guesswork with precise detection of Fc fusion protein levels at the single-cell level.
Accelerate Your CLD Pipeline
Complete high-content screens in hours, not months—reducing project timelines and enabling faster clone progression.
Prioritize High Producers Early
Rapid, quantitative readouts help you focus downstream analysis on the most productive clones—saving time and resources.
Easy Startup, Reliable Results
No optimization required—validated reagents and automation-ready design reduce variability between runs.
Compliance-Ready for Scale-Up
AOF reagents and compatibility with CHO media make the kit ideal for preclinical and GMP-aligned environments.
Maximize ROI on Each Experiment
Gain deeper insights per run—while minimizing reagent usage and hands-on time thanks to fully automated workflows.
Discover the Cyto-Cartridge®
The Cyto-Cartridge® is the core of the Cyto-Mine® platform, providing all the necessary microfluidics for implementing picodroplet workflows.
The disposable cartridge is designed to be easy-to-use, ensuring zero cross-contamination between runs and maintaining the integrity and quality of your cells for each experiment.
Cyto-Cartridges® are manufactured from fully biocompatible materials under our ISO 9001 quality management system, in a clean environment.
