Cyto-Cellect® Human IgG Kappa and Viability Assay Kit: Enhancing Cell Line Development in Cyto-Mine® Chroma

Discover how the Cyto-Cellect^® Human IgG Kappa and Viability Assay Kit enhances therapeutic cell line development by enabling real-time, multiplexed detection of viable IgG-secreting cells in Cyto-Mine^® Chroma.

Author: Romina Durigon
Senior Field Application Scientist, Sphere Bio

Keywords:  Cell line development, antibody discovery

The Cyto-Cellect® Human IgG Kappa and Viability Assay Kit is a newly launched, innovative solution designed specifically for use with the Cyto-Mine® Chroma Single Cell Analysis Platform. This cutting-edge assay enables simultaneous real-time detection of human IgG-secreting cells and the identification of apoptotic and necrotic cells across all stages—from early apoptosis to late apoptosis and necrosis. 

At the core of the kit are Cyto-Cellect® human IgGk FRET probes, which have been validated for specific binding to CHO cell-secreted human IgGk, and a customised viability dye that selectively targets apoptotic and necrotic cell membranes (Figure 1). 

Figure 1: Schematic of the Cyto-Cellect® human IgG kappa and Viability assay kit in action. Each picodroplet encapsulates a single cell, a customised viability dye (red particle), and a pair of IgG-specific fluorescent probes. Secreted IgGs from viable CHO cells form a ternary FRET complex, triggering fluorescence changes in the green and orange channels (LEFT). Meanwhile, apoptotic and necrotic cells are detected via the viability dye, which fluoresces in the far-red channel (RIGHT).