Cyto-Mine® Chroma utilizes sophisticated multiplexing to enable more accurate screening and cellular profiling.
Author: Benjamin Duckless
Senior Field Application Scientist, Sphere Bio
Keywords: Cell line development, antibody discovery
Introduction
As antibody discovery and cell line development workflows evolve, researchers need advanced tools in their quiver to aid in faster, and more accurate decision-making processes in their respective research disciplines. Cyto-Mine® Chroma, the next-generation solution from Sphere Bio, enhances the ability of detecting the top cells of interests with the addition of three addition laser and detection channels. Paired with microfluidic pico-droplet technology, the system allows for interrogation of more complex biological questions across millions of cells making it easier to select cells with high specificity and increased confidence.
This study demonstrates the accuracy of Cyto-Mine® Chroma in functional bioassays, focusing on a 4-color serial gating and sorting validation. These results highlight how advanced fluorescence detection can improve screening efficiency, particularly for complex targets like surface membrane proteins.
Key Findings
4-COLOR MULTIPLEX ASSAYS
∙ Separate and distinguish key cells based on specific fluorescent maker profiling.
∙ Serial gating delivers higher accuracy to screen and select the cell of interest.
ENCAPSULATION TECHNOLOGY enables functional screening of millions of cells in one experiment.
OFF-LINE FLUORESCENT IMAGING confirms the presence of all four fluorescent makers of sorted picodroplets that meet the selection criteria and have been dispensed into a microplate.
Study Insights
By expanding fluorescence detection to four channels and introducing serial gating during sorting, Cyto-Mine® Chroma provides a more refined approach to screening and selecting target cells of interest. The ability to distinguish multiple cell types within a single droplet streamlines discovery and development workflows and enhances assay flexibility.
Conclusion
Cyto-Mine® Chroma represents a major leap forward in single-cell analysis and antibody discovery. This study highlights its ability to improve assay resolution, increase screening efficiency, and support next-generation therapeutic development.
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Explore the data. See how Cyto-Mine® Chroma can accelerate your antibody discovery and cell line development efforts.
The Next Generation: Cyto-Mine® Chroma
Automate. Accelerate. Analyze millions of cells in a single day.
Think Cyto-Mine®, but supercharged, enabling multiplexing and greater assay flexibility to fit your needs. It means that you can examine vastly greater numbers of cells — and isolate the most valuable ones— with unparalleled precision.
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