Cyto-Mine® as a reliable platform for Antibody Discovery (AbD) workflows
Uncover the Precision of Cyto-Mine® in Antibody Discovery Workflows
Identify, Isolate, and Subclone Rare Antigen-Specific Cells with 95-100% Accuracy
In the current study we aimed to replicate a real-life scenario of an antibody discovery (AbD) workflow, where a small subpopulation of single cells secreting antibodies of interest is found within a large population of other antibody-secreting cells.
We then used Cyto-Mine®, the first fully automated integrated platform based on picodroplet technology, for identification, isolation and subcloning of these rare antigen (Ag)-specific single cells.
Furthermore, we evaluated the use of Cyto-Mine® in subcloning to enrich small populations of antigen-specific cells, thus demonstrating its application in all types of AbD workflows.
Key takeaways from this application note:
Cyto-Mine® was used to identify, isolate, and subclone rare antigen-specific cells in antibody discovery workflows.
An Ag-specific FRET assay was used to detect anti-TNFα antibody-secreting cells at the single-cell level.
Cyto-Mine® detected rare anti-TNFα cells mixed with irrelevant antibody-secreting cells, sorting and dispensing them into 96-well plates.
The platform subcloned and enriched high-performing antibody-secreting cells, significantly increasing the population of secreting cells.
Cyto-Mine® demonstrated high accuracy (95-100%) and effectiveness for antibody discovery and hybridoma-based applications.
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The Next Generation: Cyto-Mine® Chroma
Automate. Accelerate. Analyze millions of cells in a single day.
Think Cyto-Mine®, but supercharged, enabling multiplexing and greater assay flexibility to fit your needs. It means that you can examine vastly greater numbers of cells — and isolate the most valuable ones— with unparalleled precision.
