Cyto-Mine® Chroma: Accelerating Single-Cell Analysis and Sorting using Multi-Laser Picodroplet Technology
Antibody therapeutics dominate drug development; however, lengthy workflows and rising costs in their discovery and development present significant challenges. Sphere Bio’s Cyto-Mine® system leverages picodroplet technology, microfluidics, and imaging to overcome these hurdles in Antibody Discovery (AbD) and Cell Line Development (CLD), accelerating therapeutic development.
Cyto-Mine® Chroma: Next-generation multi-laser single-cell analysis
Here we introduce the next-generation Cyto-Mine® platform, Cyto-Mine® Chroma. This advanced microfluidic platform, equipped with multi-laser, single-cell analysis capabilities, enables high-throughput, multiplex functional screening of millions of cells in a single day.
Precision sorting and high-accuracy cell isolation
Our findings demonstrate that Cyto-Mine® Chroma can precisely sort and dispense cells based on fluorescence signals and sequential gating. Additionally, it achieves over 90% accuracy in isolating cells based on productivity and membrane dye from mixed populations. In an experiment simulating an AbD workflow, Cyto-Mine® Chroma successfully isolated rare cells secreting antigen-specific antibodies with high precision.
Multiplexed cell isolation without compromising performance
Furthermore, by utilizing a viability dye alongside IgG-specific probes, we demonstrated its ability to isolate CHO cells producing human IgG4 based on both productivity and viability, without compromising cell performance. These results highlight Cyto-Mine® Chroma’s robust multiplexing capabilities, making it an invaluable tool for isolating specific cell populations in both CLD and AbD workflows.
In this poster, learn how the Cyto-Mine® Chroma’s multiplexing capabilities make it invaluable for isolating specific cell population.
DOWNLOAD NOW
Cyto-Mine® Chroma: Accelerating Single-Cell Analysis and Sorting using Multi-Laser Picodroplet Technology
Keywords: Sphere Bio, picodroplet technology, biologics discovery, scale-up automation, biopharma innovation, single-cell analysis, Sphere Bio webinar, biotechnology automation, biologics production.
