Cyto-Cellect® PLUS: Detect Human IgGκ and λ with Cyto-Mine® Platforms
Discover the Versatility of Cyto-Cellect® PLUS in Detecting Human IgG
Reliably Isolate IgGκ and λ Secreting Cells with Cyto-Mine® Platforms
Cyto-Cellect® PLUS is designed to work in conjunction with our Cyto-Mine® platforms. It detects whole-molecule human IgG with κ and λ light chain, secreted from cells encapsulated within picodroplets
(Figure 1). The fluorescent probes bind to human IgG after secretion from encapsulated cells.
Using Cyto-Cellect® PLUS with Cyto-Mine® platforms provides an assay for the measurement of single cell productivity by rapidly detecting secreted IgG, enabling the identification and selection of cells with the highest productivity.
Cyto-Cellect® PLUS is certified animal origin free (AOF) and is tested for isolation of IgG secreting CHO cells (IgG4κ and IgG1λ light chain) using Cyto-Mine®. The donor and acceptor probes are verified to bind to human IgG1-4λ and human IgG1-4κ.
Figure 1. Schematic diagram of the Cyto-Cellect®PLUS detection kit for IgG secreting cells.
A customized pair of IgG-specific AOF fluorescent probes are trapped within each picodroplet. IgG secreted from the encapsulated cell is recognized by the detection probe pair forming a ternary FRET complex that induces a change in fluorescent signal.
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The Next Generation: Cyto-Mine® Chroma
Automate. Accelerate. Analyze millions of cells in a single day.
Think Cyto-Mine®, but supercharged, enabling multiplexing and greater assay flexibility to fit your needs. It means that you can examine vastly greater numbers of cells — and isolate the most valuable ones— with unparalleled precision.
