Explore how the updated fluorescent detection capabilities on Cyto-Mine® Chroma can uncover active and viable antibody secreting cells.
Author: Benjamin Duckless
Senior Field Application Scientist, Sphere Bio
Keywords: Cell line development, antibody discovery
Introduction
As antibody discovery and cell line development landscapes evolve, researchers require reliable tools at their disposal to aid in faster, more streamlined decisions. Sphere Fluidics has answered this call with the newly launched Cyto-Mine® Chroma. Outfitted with enhanced detection parameters, a new user interface and the tried-and-true picodroplet technology; the Cyto-Mine® Chroma enables the exploration of complex biological questions across millions of cells in a refined and approachable workflow.
This study presented showcases how Cyto-Mine® Chroma can be utilized to run functional bioassays requiring multiplexing to discern picodroplets containing viable antibody secreting cells from non-viable/apoptotic cells.
Figure shows: Schematic diagram for assay reaction and gating strategy during sorting.
Key Findings
VIABILITY SELECTION ASSAY
∙ Far Red Fluorescence (eFL670) – Viability dye
∙ Green Fluorescence (AF488) – FRET Donor Probe
∙ Orange Fluorescence (AF594) – FRET Acceptor Probe
ENCAPSULATION TECHNOLOGY enables functional screening by creating millions of picolitre sized reaction vessels in a single experiment.
OFF-LINE FLUORESCENT IMAGING confirms the presence of all three key components of a sorted picodroplet that meets the selection criteria and has been dispensed from the Cyto-Mine® Chroma to a microplate.
Study Insights
By expanding fluorescence detection capabilities to four channels and introducing serial gating during the sorting procedure, Cyto-Mine® Chroma provides a more refined approach to screening and selecting antibody secreting cells of interest. The utilization of a viability screening assay in conjunction with a FRET assay, takes it one step further by not only selecting cells that are actively producing antibodies, but those that are also viable and non-apoptotic.
Figure C) Gating cells during sorting. Figure D) Analysis of FRET response in live versus apoptotic cell population
Conclusion
Cyto-Mine® Chroma represents a major leap forward in single-cell analysis. This study showcases its ability to improve assay resolution, increase screening efficiency, and support next-generation therapeutic development.
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Explore the data. See how Cyto-Mine® Chroma can accelerate your antibody discovery and cell line development workflows.
The Next Generation: Cyto-Mine® Chroma
Automate. Accelerate. Analyze millions of cells in a single day.
Think Cyto-Mine®, but supercharged, enabling multiplexing and greater assay flexibility to fit your needs. It means that you can examine vastly greater numbers of cells — and isolate the most valuable ones— with unparalleled precision.
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