We’re heading to Festival of Biologics USA 2025 – Our team of experts will be on hand to showcase how our award-winning picodroplet microfluidic technology is revolutionizing single-cell analysis and isolation. Whether you’re working in antibody discovery, cell therapy development, or synthetic biology, our technology empowers you to find, analyze, and isolate the most valuable cells with unparalleled speed and efficiency.
If you’re attending, don’t miss the chance to connect with us! Book a meeting with our team to discover how our solutions can accelerate your research and streamline your workflows.
Insights and Innovations
Join John Lesnick for a Presentation and Poster Showcase
SPEAKER PRESENTATION
2:30 – 2.50pm
POSTER PRESENTATION
6:10pm (during the drinks reception)
John Lesnick
Senior Field Application Scientist
Sphere Bio
SPEAKER PRESENTATION TITLE
Using Sphere Bio’s multi-laser Cyto-Mine® Chroma, to accelerate clonal selection during therapeutic development
SPEAKER PRESENTATION ABSTRACT
Sphere Bio’s Cyto-Mine® Chroma advances antibody discovery and cell line development with microfluidics & multi-laser single-cell analysis. It enables high-throughput screening, accurately sorting cells by productivity & viability, accelerating therapeutic development.
POSTER DETAILS
Cyto-Mine® Chroma: Accelerating Single-Cell Analysis and Sorting using Multi-Laser Picodroplet Technology
POSTER ABSTRACT
Antibody therapeutics dominate drug development; however, lengthy workflows and rising costs in their discovery and development present significant challenges.
Sphere Bio’s Cyto-Mine® system leverages picodroplet technology, microfluidics, and imaging to overcome these hurdles in Antibody Discovery (AbD) and Cell Line Development (CLD), accelerating therapeutic development.
Here we introduce the next-generation Cyto-Mine platform, Cyto-Mine Chroma. This advanced microfluidic platform, equipped with multi-laser, single-cell analysis capabilities, enables high-throughput, multiplex functional screening of millions of cells in a single day. Our findings demonstrate that Cyto-Mine Chroma can precisely sort and dispense cells based on fluorescence signals and sequential gating.
Additionally, it achieves over 90% accuracy in isolating cells based on productivity and membrane dye from mixed populations. In an experiment simulating an AbD workflow, Cyto-Mine Chroma successfully isolated rare cells secreting antigen-specific antibodies with high precision. Furthermore, by utilizing a viability dye alongside IgG-specific probes, we demonstrated its ability to isolate CHO cells producing human IgG4 based on both productivity and viability, without compromising cell performance.
These results highlight Cyto-Mine Chroma’s robust multiplexing capabilities, making it an invaluable tool for isolating specific cell populations in both CLD and AbD workflows.
Visit the event website to register now
Festival of Biologics USA is America’s most exciting biologics event! Over two days, the industry’s brightest minds will gather to explore the latest breakthroughs in biologic drug development, from discovery to market access. With 300 speakers, 120 exhibitors, and 1,500 attendees, this is the place to be for cutting-edge science and collaboration.
