Cyto-Cellect® Mouse: Detection of Mouse IgG with Cyto-Mine® Platforms
Discover the Versatility of Cyto-Cellect® Mouse in Detecting Mouse IgG
The Cyto-Cellect® Mouse Assay Kit from Sphere Bio is specifically designed to be used with our Cyto-Mine® platforms.
This kit identifies whole-molecule mouse IgG secreted by cells encapsulated in picodroplets (Figure 1). Fluorescent probes in the kit bind to mouse IgG post-secretion from these encapsulated cells.
In combination with a Cyto-Mine® platform, this kit offers a rapid assay to measure single-cell productivity by detecting secreted IgG, thereby facilitating the identification and selection of cells with the highest productivity
Key Highlights:
Compatibility with Cyto-Mine® Platforms
Cyto-Cellect® Mouse can be used in combination with Cyto-Mine® platforms to allow sorting and dispensing of single cells, based on their productivity.
Short incubation time in Cyto-Mine® Platforms
Discrete FRET+ population is detectable as early as 60 minutes following incubation of cells and Cyto-Cellect® Mouse probes in Cyto-Mine® platforms. Signal intensity increases upon longer incubation.
High signal intensity & sensitivity
The kit shows good signal intensity in both plate reader as well as Cyto-Mine® platform assays, with more than 4-fold increase in FRET signal.
Low cross-reactivity
The kit offers no/low cross-reactivity with human IgG, mouse IgM, rabbit IgG and rat IgG.
Figure 1. Schematic diagram of the Cyto-Cellect® Mouse for IgG secreting cells. A customized pair of IgG-specific fluorescent probes are contained within each picodroplet. IgG secreted from the encapsulated cell is recognized by the detection probe pair forming a ternary FRET complex that induces a change in fluorescent signal.
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The Next Generation: Cyto-Mine® Chroma
Automate. Accelerate. Analyze millions of cells in a single day.
Think Cyto-Mine®, but supercharged, enabling multiplexing and greater assay flexibility to fit your needs. It means that you can examine vastly greater numbers of cells — and isolate the most valuable ones— with unparalleled precision.
