Protocol: Use of Cyto-Cellect® Mouse and Viability Probe with Cyto-Mine® Chroma
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Protocol Overview
In this protocol you will learn how to accelerate Antibody Discovery (AbD) workflows by ensuring only viable, high‑quality secreting cells are carried forward into the development pipeline.
Using the Cyto‑Cellect® Mouse assay kit in conjunction with our Viability Probe provides a powerful, multiplexed FRET‑based method enabling simultaneous detection of mouse IgG secretion and cell viability at the single‑cell level on the Cyto‑Mine® Chroma platform.
It delivers high‑throughput, automated single‑cell screening on the Cyto‑Mine® Chroma platform, improves hit quality by removing compromised cells early, and provides sensitive, specific secretion detection through donor‑acceptor FRET pairing – all of which streamline discovery, enhance downstream sequencing and expansion success, and accelerate the development of strong candidate clones.
Schematic diagram for Cyto-Cellect® Mouse and Viability Probe assay on Cyto-Mine® Chroma. This method directly evaluates secretion by using FRET, whereby the FRET donor (Alexa Fluor 488) is paired with a FRET acceptor (Alexa Fluor 594). The two FRET probes bind and form a three-molecule complex only in the presence of mouse IgG, providing sensitive and highly specific detection of secretion at single cell level. Simultaneous detection of apoptotic or dying cells by binding of the viability probe to the membrane-integrity compromised cells provides a readout for cell viability.
Have you read enough to want the full protocol?
If yes, simply click the button, and fill in the form to download the complete protocol and start using Cyto-Cellect® Mouse in combination with viability probe on Cyto-Mine® Chroma.
Otherwise, keep reading if you need a little more information about the workflow and some of the key benefits first!
How it Works:
Step 1
Prepare Cyto‑Cellect® Mouse assay and viability probe
Step 2
Single‑Cell Encapsulation:
- ASCs are encapsulated into uniform picodroplets with both probe systems
Step 3
Incubation & FRET Signal Generation:
- Measurable emission signal generated
Step 4
Gating & Sorting:
- Gate 1: Exclude non‑viable cells
- Gate 2: Include IgG‑secreting viable cells
Step 5
Dispense antigen-specific ASC for downstream analysis (e.g. RT-PCR)
Key Benefits:
1) Early Identification of Rare Clones
Capture high-value antibody-secreting cells early in the pipeline
2) Accelerated Discovery
Screen millions of cells in a single run, reducing development timelines and costs
3) Confident Selection of High Value Clones
Link secretion and viability to individual cells – ensuring only the strongest candidates advance
4) Enhanced Downstream Performance
Healthier, more productive clones yield higher success rates during cloning, expansion, and characterization
5) Compatible with High Throughput Discovery
Ideal for hybridoma and other mouse‑based IgG discovery systems
Ready for the full protocol now?
Simply click the button, and fill in the form to download the complete protocol and start combining Cyto-Cellect® Mouse with a Viability Probe to screen for viable antigen-specific cells on Cyto-Mine® Chroma.
The Next Generation: Cyto-Mine® Chroma
Automate. Accelerate. Analyze millions of cells in a single day.
Think Cyto-Mine®, but supercharged, enabling multiplexing and greater assay flexibility to fit your needs. It means that you can examine vastly greater numbers of cells — and isolate the most valuable ones— with unparalleled precision.
