Description
Overview
Confidently identify your top producing clones—fast. Cyto-Cellect® Mouse is a ready-to use, FRET-based assay that detects secreted whole-molecule mouse IgG at the bulk, or single cell level. The kit is designed for use with Cyto-Mine® technology.
The kit’s fluorescent probes are encapsulated with the cells of interest inside picodroplets. As protein secretion occurs, the probes bind to specific regions of the antibody near each other, allowing for the generation of a measurable FRET signal. The result? Rapid and highly sensitive insights into productivity.
Who is it for?
- Antibody Discovery (Abd) Teams – Select high-yielding, stable clones with confidence.
- CDMOs & BioPharma Companies – Accelerate therapeutic development.
How it works
Cyto-Cellect® Mouse uses a FRET-based detection mechanism to quantify IgG secretion from individual cells (primary mouse cells or hybridomas secreting mouse IgG). During Cyto-Mine® platform operation, each cell is encapsulated in a picodroplet alongside specific fluorescent probes (donor and acceptor probes). When a cell secretes IgG, the probes bind in close proximity, forming a ternary complex on the secreted antibody. When interrogated on the Cyto-Mine® platform, the fluorescent donor probe in this complex is excited and transfers energy to the acceptor probe resulting in a shift in fluorescent signal. This shift in signal enables for the measurement of cell productivity aiding in the clone selection process.
Diagram for mouse for IgG secreting cells. A customized pair of IgG-specific fluorescent probes are contained within each picodroplet. IgG secreted from the encapsulated cell is recognized by the detection probe pair forming a ternary FRET complex that induces a change in fluorescent signal.
Features
- FRET-Based Assay for Secreted Antibodies
Enables sensitive, real-time detection of antibody secretion from single cells encapsulated in picodroplets. - Optimized for Cyto-Mine® Platform Workflows
Seamless compatibility with CHO culture media and Cyto-Mine® platforms—no assay customization needed. - Animal-Origin-Free (AOF) Reagents
Manufactured without human- or animal-derived materials to meet regulatory and quality expectations. - Workflow-Ready Reagent Kit
Includes 5 premixed vials for off-the-shelf ready assays - Binds IgG1, IgG2a and IgG2b isotypes
Benefits
- Confident Clone Selection
Detect only cells secreting complete antibodies—boosting reliability in selecting high-producing clones. - Accelerated Development Timelines
Streamline screening and shorten time-to-decision with high-throughput, single-cell resolution data. - Assay Simplicity = Faster Startup
Skip lengthy optimization—validated probes work out of the box, reducing setup time and variability. - Improved Compliance Readiness
AOF formulation helps support traceability and regulatory alignment for GMP and CDMO partners. - Greater Insight, Less Reagent Waste
Multiplex detection in nanoliter volumes provides more data per cell while minimizing assay costs.
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